With use of multiplex real-time PCR technology, this in-vitro diagnostic kit can detect the presence or absence of RNA focusing on 2 targeting genes: ORFlab and N gene. ORFlab and N gene signals can be amplified and detected based on the designed Taqman probes of those target genes during the amplification process. B2M RNA gene is also included for each sample to ensure specimen validity from specimen collection and RNA extraction, and monitor PCR amplification procedure avoiding false negative results.